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Resting Em is best measured with microelectrodes filled with 3 M K-acetate. Fibers were prepared and tested, according to protocol 2, except that the physiological solution contained 1% FBS. Experimental temperature: 37°C. Microelectrodes were filled with KCl, K-citrate, or K-acetate. Error bars represent means ± SE of four to seven fibers from three to four mice. §Mean membrane potential (Em) was significantly different from the mean at time 0; using ANOVA and LSD, P < 0.05.
