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(A) Structures of 5a–d. (B) Effect of substitutions at residue 2 on the agonistic activity in CHO cells toward stably expressed human NMUR1 (black bar) and NMUR2 (gray bar) as determined by the calcium mobilization assay. Peptide concentrations, 0.1 and 1 nM; positive control, hNMU (activity at 1000 nM = 100%).
