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. 2015 Jan 25;6(3):260–265. doi: 10.1021/ml500385d

Table 3. HPLC Analyses of the Enzymatic Assays of l-Tryptophan and Derivatives with rhIDO (Retention Times and MS).

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a

Retention times determined by HPLC analyses (or UPLC analyses; see Supporting Information). Elution conditions: CH3CN/ammonium acetate (10 mM), 10/90 (v/v), flow 0.7 mL/min.

b

UV detection (method A) at 221 and 284 nm.

c

UV detection (method A) at 321 or 360 nm.

d

Mass spectra (see Supporting Information).

e

Retention times determined by HPLC analyses (or UPLC analyses; see Supporting Information). Elution conditions: CH3CN/ammonium acetate (10 mM), 5/95 (v/v), flow 0.7 mL/min.

f

Racemate. n.o.: not observed because hydrolysis of the formyl substituent was too fast in these conditions.