Table 4. In vitro Enzymatic Assays Performed with rhTDO (37 °C, pH 7.5).
percentage of substrate
consumed after 1 h of incubation |
||
---|---|---|
substrates tested | rhTDO 1 μM | rhTDO 10 μM |
l-Trp (11) | 67%a | 100%a |
1-Me-l-Trp (12) | 0%a | 3%a |
5-HO-l-Trp (13) | 0%a | 14%a |
1-FETrp (5)b | 0%a | 2%a |
Substrate concentration: 100 μM.
Racemate.