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. 2015 Mar 15;142(6):1102–1112. doi: 10.1242/dev.116277

Fig. 7.

Fig. 7.

Magi is required for Baz recruitment. (A-F″) 24-h APF pupal eye discs in different mutant backgrounds. The yellow lines (A′,F′,F″) mark the boundaries between wild-type and mutant tissues. (A-A″) In Magi mutant clones marked by the absence of β-galactosidase (red, A″), Baz (green, A′) is less cortical and accumulates in the cytoplasm. (B-D) In a whole Magi mutant background, Baz (white) is absent from the membrane in IOCs (B). Whereas cortical Baz localization is completely rescued by providing a Magi transgene (C), only very faint cortical recruitment of Baz could be observed with a mutant MagiΔWW transgene. (E-E″) In whole RASSF8 mutant discs, Baz (green, E′) is very weakly associated with the cortex, whereas Magi localization (red, E″) is unaffected. (F-F″) In ASPP mutant clones marked by the absence of β-galactosidase (red), Baz (green, F′) is weaker at the membrane, in particular between IOCs [Baz at membrane in ASPP versus wild type=0.86; P=0.005 (paired t-test)], whereas Baz localization in primary pigment cells is less affected. Membranes are highlighted by E-Cad (blue, F″). Scale bar: 5 µm.