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. 2015 Jan 29;18(3):pyu071. doi: 10.1093/ijnp/pyu071

Figure 3.

Figure 3.

Fear conditioning induces sonic hedgehog (Shh) expression in the basolateral amygdala (BLA). (A) Mice were given 15 tone-footshock pairings, and the expression of the Shh protein in the BLA was determined by Western blotting analysis at 1, 3, and 7 days after conditioning. *p < 0.05, **p < 0.01 vs. naïve (n = 5–6 mice/group). (B) Western blotting analysis of Shh protein levels in BLA of naïve, unpaired, and paired mice at 3 days after conditioning. *p < 0.05 vs. naïve (n = 5–6 mice/group). (C) Representative images of Shh-immunoreactive cells and 4’,6-diamidino-2-phenylindole (DAPI) nuclei in the BLAs of naïve unpaired and paired mice at 3 days after conditioning. (D) Quantification of Shh-positive cells in the BLAs of naïve, unpaired, and paired mice at 3 days after conditioning. *p < 0.05 vs. naïve and unpaired (n = 3–5 mice/group).