Figure 5.

Paliperidone effects on the potential regulatory mechanisms of acute and chronic stress-induced toll-like receptor-4 (TLR-4) activation II. Messenger RNA (mRNA) and protein levels of HSP70 (A and B), HMGB1 (C and D), and HSP60 (E and F) in vehicle (Veh) + no stress (C), 1mg/Kg paliperidone (P₁) + no stress, and animals exposed to acute restraint stress (6 hrs) with (P₁) or Veh paliperidone pre-treatment. Messenger RNA (mRNA) relative levels of HSP60 (G) and HSP70 (H) in Veh + no stress (C), 1mg/Kg Paliperidone (P₁) + no stress, and animals exposed to chronic restraint stress (6 hrs/day during 21 days) with (P₁) or Veh Paliperidone pre-treatment. The densitometric data of the respective bands of interest are normalized by β-actin (lower band). *p < 0.05, **p < 0.01 vs. control; ^# p < 0.05 vs. stress. One-way analysis of variance followed by Newman–Keuls post-hoc test. Data represent the mean ± standard error of the mean.