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. 2015 Jan-Feb;8(1):18–23.

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© 2014 Cancer Research Center, Shahid Beheshti University of Medical Sciences

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The stimulation calculated indexes for different vaccinated groups; C57BL/6 mice were injected subcutaneously with TC-1 cells. After two week, the mice were immunized intramuscularly (IM) twice at a two weeks interval with 100 ml phosphate buffered saline (PBS; negative control), 100 mg naked DNA vaccine encoding pcDNA3 (negative plasmid control), pcDNA3/L1, pcDNA3/E6, pcDNA3/E7, pcDNA3/E6 & pcDNA3/E7, pcDNA3/E6 & pcDNA3/L1, pcDNA3/E7 & pcDNA3/L1 and pcDNA3/E6 & pcDNA3/E7 & pcDNA3/L1 in PBS. Two weeks after final immunization, spleen of individual mice (three/group) was removed and lymphocyte proliferation was evaluated using the MTT method. Formazan crystal formation after incubation with MTT was determined by solving the crystals in DMSO, and the OD was read at 540 nm. Lymphocyte proliferation in the pcDNA3/E6 & pcDNA3/E7 & pcDNA3/L1 group was significantly higher than in the other groups especially negative control group (p<0.05).