Figure 2.

Dose–responses of select screening compounds in the Rtt109 HTS and an assay interference counter-screen. Shown are representative examples from chemotypes 1, 2, 3, 4, and 6, which displayed promising low micromolar IC₅₀ values by the primary HTS assay (solid lines). A counter-screen that replaced the acetyl-CoA substrate with the CoA reaction product produced similar dose–response curves by the same assay readout (dashed lines). Data are mean ± SD for three replicates.