Table 3. 1,2,4-Thiadiazole Series^e.

^aHTS refers to IC₅₀ values calculated from the CPM-based Rtt109 HTS method, CoA-CPM refers to IC₅₀ values calculated from the CoA-based HTS counter-screen.

^bHTS reaction aliquots from compounds tested at 125 μM final concentrations; Yes (“Y”), No (“N”) or Partial (“P”).

^cCompounds flagged as quenchers if greater than 20% assay signal reduction at 10 μM final concentrations.

^dCompounds flagged if fluorescence intensity greater than 20% assay signal at 125 μM final concentrations; compounds tested in either HTS buffer (“Buffer”), HTS buffer plus 20 μM CPM (“+ CPM”), or HTS buffer plus 7.5 μM CoA (“+ CoA”).

^eIC₅₀ values shown are means ± SD for three replicates.