Table 1. Specificity, Source, and Other Properties of the Most Commonly Used Lectinsa.
lectin | specificity | source | source type | Mw (kDa) | pI | SUc | glycoprotein | metal ions |
---|---|---|---|---|---|---|---|---|
AAL | α-l-Fuc | Aleuria aurantia | F | 72 | 9 | 2 | ||
Con A | α-d-Man, α-d-Glc | Canavalia ensiformis | P | 104 | 6.3–7 | 4 | Ca2+, Mn2+ | |
MAA-I | Gal-β-(1–4)- GlcNAc | Maackia amurensis | P | 130 | 4.7 | 2 | yes | |
MAA-II | Neu5Ac-α-(2–3)β-Gal | Maackia amurensis | P | 130 | 4.7 | 2 | yes | |
PHA-L | branched β-(1–6)GlcNAc | Phaseolus vulgaris | P | 126 | 4.2–4.8 | 4 | yes | Ca2+, Mn2+ |
PHA-E | oligo Man | Phaseolus vulgaris | P | 126 | 6–8 | 4 | yes | Ca2+, Mn2+ |
SNA-Ib | Neu5Ac-α-(2–6)Gal | Sambucus nigra | P | 140 | 5.4–5.8 | 4 | yes | |
SNA-II* | Gal, GalNAc | Sambucus nigra | P | |||||
RCA-I/RCA120 | β-d-Gal | Ricinus communis | P | 120 | 7.8 | 2 | yes | |
RCA-II/RCA60 | Gal-β-(1–4)- GalNAc | Ricinus communis | P | 60 | 7.1 | 1 | yes | |
UEA-I | α-l-Fuc | Ulex europaeus | P | 63 | 4.5–5.1 | 2 | yes | Ca2+, Mn2+, Zn2+ |
WGA | β-d-GlcNAc | Triticum vulgaris | P | 36 | >9 | 2 | no | Ca2+ |
Table based on ref (888).
SNA (EBL-elderberry bark lectin) is often available only as SNA without any specification, despite the different binding abilities of the two isolectins.
F, fungi; P, plant; SU, subunits; Gal, galactose; GalNAc, N-acetylgalactosamine; Glc, glucose; GlcNAc, N-acetylglucosamine; Fuc, fucose; Man, mannose; Neu5Ac, N-acetylneuraminic acid.