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. 2004 Jun 3;5:3. doi: 10.1186/1471-2199-5-3

Figure 1.

Figure 1

Temperature-dependent accumulation of transcripts for HSP83 and HSP70. RNA samples were prepared from promastigotes grown at 26°C (lanes 1) or incubated for 2 h at 37°C (lanes 2), 39°C (lanes 3), or 41°C (lanes 4), and analyzed by Northern blotting. Northern blots were hybridized with the L. infantum HSP83 3'-UTR (panel A). The same blot was stripped and rehybridized with probes for the L. infantum HSP70 3'-UTR-I (panel B) and the L. infantum HSP70 3'-UTR-II (panel C) [9]. Panel D shows an ethidium bromide staining of the RNA samples loaded on agarose gel prior to transfer.