Table 5.

Effect of DFMO on proliferative index of SCCs. Tumor-bearing K6/ODC transgenic mice of founder line 39/1m were administered 1% DFMO in their drinking water and sacrificed at the indicated times after the start of treatment. Control animals (n = 4) received regular drinking water and were sacrificed at various times throughout the 8 day experimental period. One hour before sacrifice, mice were injected i.p. with BrdUrd (100 μg/g body weight), and tumors and surrounding skin were processed for immunocytochemistry as described in Methods. Results represent counts of > 800 cells/section with 3–5 tumors per group.

Group	No. of Cells Counted	% of Nuclei, BrdUrd-Positive (mean ± SE)	P Value*
Control	4375	28.7 ± 3.6
DFMO, 1d	4696	21.2 ± 3.9	0.13
DFMO, 2d	4339	7.8 ± 1.0	0.007
DFMO, 4d	6202	13.3 ± 1.9	0.004
DFMO, 8d	2651	14.4 ± 1.9	0.009

*vs control value (ANOVA)