Figure 4. CLCA1 colocalizes with TMEM16A and increases TMEM16A surface expression.
(A–D) Membrane (WGA) or immunostaining of HEK293T cells transfected with pHLsec vector; (E–H), or with CLCA1. Surface TMEM16A is greatly increased by expression of CLCA1. (I–L) Membrane (WGA) or immunostaining of HEK293T cells cultured in conditioned media from cells transfected with pHLsec vector; (M–P) or cells cultured in conditioned media from cells transfected with CLCA1. TMEM16A surface expression is greatly enhanced after cells are exposed to secreted CLCA1.