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. 2015 Mar 16;9:75. doi: 10.3389/fnins.2015.00075

Figure 3.

Figure 3

Arachnoid blood-CSF barrier, subarachnoid space, pial surface, and glial end feet layers double immunolabeled with antibodies against YKL-40 and SSEA-4 (A–C) in parietal cortex of a 15th wpc human fetus. Parietal cortex of a 15th wpc fetus, immunolabeled with anti-YKL-40 (A) and anti-SSEA-4 (B), counterstained with DAPI and merged (A1,B) in (C). The images are representations of a stacked series of 10 steps, with 1 μm intervals, processed as maximum projection intensity. The staining of the end feet layer (EFL) shows multiple delicate and curled end feet membranes. YKL-40 immunoreactivity is also present in the arachnoid blood-CSF barrier (aB-CSFB in (A) and in leptomeningeal cells in the subarachnoid space (arrows in A1). In (B) the pattern of SSEA-4 distribution is seemingly equal to that of YKL-40, and the merged image of the stacked images confirms the co-localization of YKL-40 and SSEA-4 in leptomeningeal cells within the subarachnoid space (SAS) and in the end feet layer of the radial glial cells (C). Scale bar: 10 μm.