Table 1.
List of primary antibodies.
Primary antibodies | Host IgG | Dilution | HIER | Producer | Code number |
---|---|---|---|---|---|
BLBP | Rabbit IgG | 1:2000 | - | Millipore | ABN14 |
Claudin-11/OSP | Rabbit IgG | 1:1500 | - | Abcam | ab53041 |
Collagen 1 | Rabbit IgG | 1:500 | citrate, pH6 | Abcam | ab292 |
EAAT1 | Rabbit IgG | 1:400 | TEG, pH9 | Abcam | ab416 |
GFAP | Rabbit IgG | 1:10000 | TEG, pH9 | Dako | Z0334 |
IL-13Rα2 | Rabbit IgG | 1:100 | - | Proteintech | 11059-1-AP |
MAP2 | Clon HM-2 | 1:10000 | TEG, pH9 | Sigma Aldrich | M4403 |
SSEA-4 | Mouse IgG3 | 1:50/1:100 | - | Millipore | MAB4304 |
YKL-40 | Rabbit IgG | 1:10 | - | Proteintech | 12036-1-AP |
YKL-40 | Mouse, IgG2kb | 1:50/1:100 | - | * | 201.F9 |
HIER, Heat Induced Epitope Retrival. TEG, TRIS + ethylene glycol tetraacetic acid buffer. Producers, Abcam, Cambridge, United Kingdom; Millipore, Merck Life Science, Hellerup, Denmark. Dako, Glostrup, Denmark. Sigma-Aldrich, St. Louis, MO, USA. Proteintech, Manchester, United Kingdom.
Kind gift from Prof. Paul A. Price, UCSD, USA.