Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Mar 16;5:9144. doi: 10.1038/srep09144

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2015, Macmillan Publishers Limited. All rights reserved

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

PMC Copyright notice

(A) Survival of retinal microglia was assessed following 12 hours incubation in increasing concentrations of 7KCh or cholesterol using a MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. Exposure to concentrations of 7KCh > 16 μM resulted in a concentration-dependent decrease in MTT levels, indicating decreased cellular survival, while exposure to cholesterol (0–36 μM) had no significant effect. (* indicates comparisons to HPBCD-only control for which p < 0.05, 1-way ANOVA with Dunnett's multiple comparison test, n = 6–12 replicates). (B) Incubation of retinal microglial with 7KCh (black bars) at concentrations of 24 μM and 36 μM induced significant increases in the proportion of TUNEL+ cells while similar concentrations of cholesterol (gray bars) did not (* indicates comparisons to HPBCD-only control for which p < 0.05, Kruskal-Wallis test with Dunn's multiple comparison test, n = 3–9 replicates). (C) Measurements of microglial proliferation, measured by quantifying the proportion of cells incorporating BrdU, were not significantly altered by incubation with either cholesterol or 7KCh (4–12 μM).