Figure 3. Near-UV Circular Dichroism (A, C) and far-UV Circular Dichroism (B, D) spectra of wild-type and mutants thereof (A, B) and of the 3M variant and mutants thereof (C, D) recorded at 25.0 ± 0.1°C in 50 mM MOPS buffer, pH 7.0 (near-UV spectra) or 5 mM MOPS buffer, pH 7.0 with 15 mM NaCl (far-UV spectra).