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. 2015 Mar 16;6:48. doi: 10.3389/fneur.2015.00048

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Copyright © 2015 Elder, Gama Sosa, De Gasperi, Stone, Dickstein, Haghighi, Hof and Ahlers.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Altered collagen IV immunostaining in the microvasculature of blast-exposed rats. Shown are sections from a rat sacrificed 10 months after receiving three 74.5 kPa blast exposures delivered on consecutive days. (A) shows a coronal section stained with hematoxylin and eosin. (B,C) show adjacent sections immunostained with collagen IV without (B) or with pepsin (C) treatment. Immunostaining was performed as described in Gama Sosa et al. (57). Pepsin treatment (C) unmasks widespread collagen IV immunostaining. Arrow in (B) indicates a region that shows collagen IV immunostaining without pepsin pretreatment. Scale bar: 1 mm.