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. 2015 Mar 16;6:48. doi: 10.3389/fneur.2015.00048

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Copyright © 2015 Elder, Gama Sosa, De Gasperi, Stone, Dickstein, Haghighi, Hof and Ahlers.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Altered collagen IV immunostaining around blast-induced shear-related lesion. Shown are sections from a rat sacrificed 10 months after receiving three 74.5 kPa blast exposures (A–B) or a non-blast exposed control (C–D). Sections were immunostained for collagen IV without pepsin pretreatment (A,C) and counterstained with 4’,6-diamidino-2-phenylindole (DAPI) (B,D) as described in Gama Sosa et al. (57). A focal blast-induced lesion (indicted by asterisks) is apparent in (A,B). Note the vascular staining with collagen IV in the blast-exposed animal despite the lack of pepsin treatment (A) in comparison to the unstained control (C). Scale bar: 250 μm.