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. Author manuscript; available in PMC: 2015 Sep 13.
Published in final edited form as: Nat Commun. 2015 Mar 13;6:6610. doi: 10.1038/ncomms7610

Figure 4. Multiple Tup1- and transcription-related proteins are sumoylated during amino acid starvation.

Figure 4

(a) Hht1-HA, Gcn5-HA, Cti6-HA, Gal11-HA and Cyc8-HA IPs of untreated (control), or SM-treated cells were analyzed by HA and yeast SUMO (Smt3) western blots. (b) Analysis of HA IPs obtained from untreated control and SM-induced cells expressing Hht1-WT Flag and either Tup1-WT HA or K270R HA. The asterisk indicates the HA antibody light chain. (c) Analysis of Flag IPs obtained from untreated control and SM-induced cells expressing Hht1- WT Flag and either Tup1 WT-HA or K270R-HA.