Figure 1.

Experimental protocol for the sample selection. (A) Model and time-points selected. Initial progression period; initiation stage over 24 h and 7 days and promotion stage over 11 and 16 days. The preneoplastic progression period includes the evolution of persistent preneoplastic lesions over 30 days, and 5 and 9 months. The cancer progression period includes the growth of hepatocellular carcinomas over 9, 12 and 18 months. (B) Sample analysis. For tissue gene expression analysis, whole homogenates were used of the sample at 24 h and 7, 11 and 16 days. Representative samples of (B) 30 days and (C) 9 months after treatment are presented to illustrate the enrichment of samples. White arrows, adjacent tissue; black arrows, (B) preneoplastic lesions or (C) tumor lesion.