Figure 7. Gene expression analysis of qNSCs and aNSCs reveals distinct molecular signatures.
(A) Volcano plot of differentially expressed probesets in qNSCs and aNSCs. Probes have at least 2-fold change in expression and a corrected p-value < 0.05. See also Table S1.
(B–C) Pie charts showing representative GO categories for differentially expressed probesets in qNSC and aNSC (as determined in A). See also Table S2.
(D) Gene Set Enrichment Analysis (GSEA) for qNSCs vs aNSCs. Sets have FDR < 0.05 and are hand-curated into thematic categories. See also Table S3.
(E–F) Percentage of overlap with signatures of quiescent and dividing stem cells from other organs: long term (LT)/quiescent signatures (G) and short term (ST)/proliferative signatures (H) as determined by fold-change analysis of published lists compared to qNSC and aNSC populations. Qui: Quiescent, HSC: Hematopoietic Stem Cells, qMuSC: Quiescent Muscle Stem Cells, qBulgeSC: Quiescent Bulge Stem Cells, qISC: Quiescent Intestinal Stem Cells. See also Table S6.
(G–J) Targeted GPCR ligand screen. See also Table S7.
(G) Quantification of qNSC activation (fold change in % Nestin+ clones) as compared to controls (empty dots). For complete screening panel, see Figure S8L.
(H) Quantification of percentage of MCM2+ cells within activated Nestin+ clones.
(I) Quantification of aNSC clones (fold change of % clones that underwent division) as compared to controls (empty dots).
(J) Quantification of total MCM2+ aNSCs. n=3, *p<0.05, ***p<0.001.