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. Author manuscript; available in PMC: 2015 Mar 16.
Published in final edited form as: J Proteome Res. 2013 Oct 28;12(12):5463–5474. doi: 10.1021/pr400334k

Figure 2. Membrane-associated proteins with predicted export signal peptides or transmembrane domains.

Figure 2

The primary amino acid sequence of the 2,203 total proteins identified in the membrane fractions of MTB and BCG were analyzed for the presence of aminoterminal export signal peptides or transmembrane domains. A total of 440 proteins contain probable transmembrane domains predicted by the TMHMM 2.030 (omitting TMHMM transmembrane predictions likely representing N-terminal signal peptides). Additionally, 140 proteins are predicted by SignalP31 or TatP32 to contain either Sec or Tat export signal peptides, which would target them for export out of the cytoplasm by conserved bacterial secretion machinery. Of the 140 proteins with putative export signals, 54% are possible lipoproteins with lipobox motifs in their signal peptides predicted by LipoP34 or by using a lipoprotein pattern search against the M. tuberculosis H37Rv genome35.