Figure 1. Guanabenz protects differentiated rat oligodendrocyte progenitor cells from IFN-γ-mediated death and prolongs the integrated stress response.

(a) Quantification of dOPCs stained with PI/FDA to identify dead/live cells after 48 h of differentiation. Data represent three individual dOPC isolations, each with three replicates per group. (b) IFN-γ-mediated apoptotic death was confirmed via TUNEL staining of dOPCs 48 h after differentiation. N=3. (c) Western blot analysis of dOPCs treated continuously with IFN-γ alone or IFN-γ+5.0 μM guanabenz and probed with p-eIF2α, a marker of ISR activity, and eIF2α. (d) Quantification of extended time points in a western blot. Blot represents one of four individual isolations; graph represents the average of four isolations. Mixed results ANOVA (a), unpaired t-test (b,d), *P<0.05, **P<0.005, ***P<0.0005. Data are presented as mean±s.e.m.