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. 2015 Mar 13;6:6532. doi: 10.1038/ncomms7532

Figure 2. Guanabenz decreases IFN-γ-induced hypomyelination in rat cerebellar slice cultures.

Figure 2

(a–e) Anti-MBP staining of myelinated fibres (a, arrowheads) in slice cultures that were (a) untreated, (b) treated with IFN-γ or (c–e) concomitantly treated with IFN-γ and 2.5, 5.0 or 10.0 μM guanabenz. Images representative of two or three slices per treatment; the experiment was performed twice. (f) Electron microscopy analysis of slice cultures. Note the significant increase in the number of myelinated axons (arrowheads) when guanabenz and IFN-γ were concomitantly added to slices. Myelinated axons per field were determined by analysis of a minimum of 200 axons per condition. (g) Toluidine blue staining of slices left untreated, treated with IFN-γ alone or treated with IFN-γ and guanabenz. Images represent a minimum of three sections per treatment. Unpaired t-test, *P<0.05. Data are presented as mean±s.e.m. Scale bars, 100 μm (a–e), 2 μm (f) and 20 μm (g). WM: white matter, GL: granule cell layer, PCL: Purkinje cell layer.