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Hmgcr and Dhcr7 are highly expressed in Neuro2a cells. Total RNA was isolated from Neuro2a cells and analyzed by Northern blotting for the expression of cholesterogenic enzymes. The same membrane was probed sequentially with Hmgcr-, Dhcr7-, and Gapdh-32P-dCTP-labelled cDNA probes. The experiment was performed in triplicate. Note that Hmgcr and Dhcr7, the first and the last enzymes in the cholesterol biosynthesis pathway, are highly expressed in Neuro2a neuroblastoma cells.
