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. 2015 Feb 25;17(1):27. doi: 10.1186/s13058-015-0529-8

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© Singh et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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The lead compounds affect the viability of oestrogen receptor-alpha-positive cells. Dose–response curves (0.2 to 50 μM) of the lead compounds showing decrease in cell viability as assessed by 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulphophenyl)-2H-tetrazolium assay. The compounds (A) VPC-13002, (B) VPC-16225 and (C) VPC-16230 inhibited the growth of oestrogen receptor-alpha (ERα)-positive MCF7 and tamoxifen-resistant (TamR) cells (TamR3 and TamR6 cell lines) with very little effect on ERα-negative MDA-MB-453 and HeLa cells, except VPC-13002, which was toxic in both the control cell lines. Data points represent average of two independent experiments performed in triplicates. Error bars indicate standard error of mean for n = 6 values. Data were fitted using GraphPad Prism 5 software to calculate the log of concentration of the inhibitors versus percentage growth.