Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Feb 25;17(1):27. doi: 10.1186/s13058-015-0529-8

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© Singh et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

PMC Copyright notice

The lead compounds inhibit mRNA and protein expression levels of oestrogen receptor-alpha-dependent genes. (A) VPC-16230 significantly decreased mRNA levels of pS2, cathepsin D and cell division cycle 2 (CDC2) at 12 and 6 μM in the presence of 1 nM oestradiol (E2) in MCF7 cells. Data points represent average of two independent experiments performed in triplicates. Error bars indicate standard error of mean for n = 6 values. A P-value <0.05 was considered significant (*) compared with E2 + dimethyl sulphoxide (DMSO) control. (B) VPC-16230 did not significantly decrease mRNA levels of pS2, cathepsin D and CDC2 at 12, 6 and 3 μM in the absence of 1 nM E2. (C) VPC-16230 decreased protein levels of pS2, cathepsin D and CDC2 at 12 and 6 μM in MCF7 cells as observed by Western blot analysis. GAPDH, Glyceraldehyde 3-phosphate dehydrogenase; OHT, 4-Hydroxytamoxifen.