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. Author manuscript; available in PMC: 2015 Mar 16.
Published in final edited form as: J Biol Chem. 2006 May 3;281(27):18684–18690. doi: 10.1074/jbc.M600733200

FIGURE 1. ChIP assays with antibody against IKKα (A) or RelA (B) in endotoxin-stimulated RAW cells.

FIGURE 1

RAW cells were treated with endotoxin (100 ng/ml) for various time points (0–1 h), IKKα and RelA were immunoprecipitated with specific antibody, and binding to the promoter region of various pro-inflammatory cytokine genes was detected by PCR primers for COX-2, MnSOD, RANTES, and IκBα, which flank a κB binding motif. Binding to the various promoters is compared with PCR of the input DNA against using the COX-2 primers. LPS, lipopolysaccharide.