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. Author manuscript; available in PMC: 2015 Mar 16.
Published in final edited form as: J Biol Chem. 2006 May 3;281(27):18684–18690. doi: 10.1074/jbc.M600733200

FIGURE 2. The subcellular localization of RelA and IKKα was detected in nuclear and cytoplasmic protein extracts from RAW cells by immunoblot (IB).

FIGURE 2

Antibodies specific for cytoplasmic α-tubulin and nuclear HDAC1 demonstrate separation of the nuclear and cytoplasmic protein fractions. RAW cells were treated with 100 ng/ml endotoxin for 0 – 60 min.