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. Author manuscript; available in PMC: 2015 Mar 16.
Published in final edited form as: J Biol Chem. 2006 May 3;281(27):18684–18690. doi: 10.1074/jbc.M600733200

FIGURE 4. A FLAG-tagged IKKα or a kinase-inactive mutant (IKKα-KA) was transiently expressed in 293 cells for 24 h and immunoprecipitated (IP) with anti-FLAG antibody.

FIGURE 4

Captured IKKα and IKKα-KA was combined with recombinant histone H3 and [γ-32P]ATP. We used the two types of expression vector, wild-type IKKα and mutant IKKα(K44A), which contained an alanine substitution of a conserved lysine residue in its kinase domain. IB, immunoblot.