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. Author manuscript; available in PMC: 2015 Mar 16.
Published in final edited form as: J Biol Chem. 2006 May 3;281(27):18684–18690. doi: 10.1074/jbc.M600733200

FIGURE 5.

FIGURE 5

A, RAW cells were treated with endotoxin for various time points. The nuclear protein was extracted and blotted with anti-NIK, RelA, HDAC1, and α-tubulin antibodies. The whole cell lysate was also blotted with anti-NIK to show the total amount of NIK. B, RAW cells were cultured in glass bottom microwell dishes (MatTek) and transfected with GFP-NIK. After 24 h, the cells were stimulated with lipopolysaccharide (LPS)(100 ng/ml). The live image of GFP fluorescence was taken at 5-min intervals by confocal microscopy. During this process, the cells were kept in 37 °C and phenol red-free media. The intensity of fluorescence in the nucleus was quantified with a Zeiss LSM image viewer (Zeiss). IB, immunoblot; LMB, leptomycin B.