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. Author manuscript; available in PMC: 2015 Sep 11.
Published in final edited form as: Cell Rep. 2014 Aug 28;8(5):1461–1474. doi: 10.1016/j.celrep.2014.07.053

Figure 3. PTEN-AKT-mTORC1 Signaling Regulates HK2 Protein Expression.

Figure 3

(A–D) HK2 mRNA (A, C) and HK1 mRNA (B, D) expressions in MEFs and in prostate with the indicated genotypes were examined by real time RT-PCR, respectively. (E–P) Effects of knocking down AKT or Raptor on HK2 expression: HK2 protein expression was determined by Western blot in PtenΔ/Δp53Δ/Δ MEFs with depletion of AKT (E) or Raptor (F), in mouse PtenΔ/Δp53Δ/Δ prostate cancer cells with depletion of AKT (I) or raptor (J), and in human Pten/p53-deficient prostate cancer PC3 cells with depletion of AKT (M) or Raptor (N). HK2 mRNA expression was detected by real time RT-PCR in AKT- or Raptor-depleted Pten/p53-deficient MEFs (G and H), in AKT- or Raptor-depleted Pten/p53-deficient mouse prostate cancer cells (K and L), and in AKT- or Raptor-depleted Pten/p53-deficient human prostate cancer cells (O and P). For Western blots, tubulin was used as a loading control. All data are means ± SEM. *P ≤ 0.01. ns: not statistically significant. See also Figure S6.