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. Author manuscript; available in PMC: 2015 Mar 16.
Published in final edited form as: Nat Commun. 2014;5:3110. doi: 10.1038/ncomms4110

Figure 4. Proximity of Cys56 and horizontal helix is required for electron transfer.

Figure 4

Inserting residues between Cys56 and the horizontal helix lowers the rate of electron transfer, which is initiated by RNaseA reduction of the Trx domain (red bars). The DTT reduction at the active site (blue bars) is largely unchanged. Error bars represent standard error of three duplicated measurements. Mutants with insertion between Cys56 and the horizontal helix (orange box) were made by either moving Cys56 (red) to a different position on the polypeptide chain through mutations (Gly55Cys/Cys56Ala, Gly54Cys/Cys56Ala), or by inserting a flexible linker (Gly or Gly-Gly-Ser-Gly-Ser) between Cys56 and Gly57. The mutated residues are shown in blue and the inserted residues in green.