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. Author manuscript; available in PMC: 2015 Jun 10.
Published in final edited form as: Sci Transl Med. 2014 Dec 10;6(266):266ra170. doi: 10.1126/scitranslmed.3010189

Fig 2. VIS and DPU effects on doxorubicin-induced cardiac cell death in vitro and in vivo.

Fig 2

Doxorubicin treated neonatal rat cardiomyocytes were treated with VIS and DPU and assessed for apoptosis with TUNEL (A) or AnnexinV (B) staining. (C) Doxorubicin treated HL1 cells were treated with VIS or DPU and assessed for apoptosis with Annexin V staining. (D) Viability of HL1 cells treated with doxorubicin and VIS or DPU. Effect of VIS or DPU on cardiac cell death in doxorubicin treated zebrafish (E) and mice (F) as measured by TUNEL assay. n = 4–5 mice per group. (G) Effect of VIS on doxorubicin-induced apoptosis in heart as assessed by ex vivo imaging of the whole heart with the dye AnnexinV-750. n = 5 mice per group, compared using one-way ANOVA and the Tukey post-test. DM – DMSO treated control samples, Dox – doxorubicin treated samples, Dox + VIS – doxorubicin and VIS co-treated samples, Dox + DPU – doxorubicin and DPU co-treated samples. Statistics (compared to doxorubicin treated samples): * p< 0.05, ** p<0.01, and *** p<0.001.