Table 1.
Analysis of Mitochondrial Electron Transport Chain
| Substrate | Respiration rate (μatom O2/min/mg protein) | ADP/O ratio |
|---|---|---|
| Col-O | ||
| Succinate | 8.96±0.44 | 1.34±0.06 |
| +Rotenone | 8.91±0.39 | 1.36±0.03 |
| +Antimycin A | 0.23±0.1 | — |
| +Cyanide | 0.1±0.0 | — |
| Ascorbate/TMPD | 7.58±0.29 | 1.30±0.05 |
| +Rotenone | 7.54±0.37 | 1.31±0.08 |
| +Antimycin A | 7.25±0.38 | 1.32±0.08 |
| +Cyanide | 0.0±0.0 | — |
| hrl1 | ||
| Succinate | 6.40±0.31 | 1.36±0.07 |
| +Rotenone | 6.32±0.28 | 1.34±0.06 |
| +Antimycin A | 0.27±0.2 | — |
| +Cyanide | 0.0±0.0 | — |
| Ascorbate/TMPD | 5.35±0.24 | 1.33±0.05 |
| +Rotenone | 5.31±0.26 | 1.32±0.03 |
| +Antimycin A | 5.74±0.28 | 1.34±0.04 |
| +Cyanide | 0.16±0.05 | — |
| HRL1 OX 1 | ||
| Succinate | 18.69±0.93 | 1.37±0.05 |
| +Rotenone | 18.43±0.91 | 1.37±0.03 |
| +Antimycin A | 0.90±0.1 | — |
| +Cyanide | 0.0±0.05 | — |
| Ascorbate/TMPD | 13.65±0.87 | 1.36±0.05 |
| +Rotenone | 13.45±0.88 | 1.35±0.03 |
| +Antimycin A | 13.32±0.92 | 1.36±0.03 |
| +Cyanide | 0.0±0.0 | — |
| HRL1 OX 2 | ||
| Succinate | 18.8±0.83 | 1.34±0.05 |
| +Rotenone | 18.54±0.86 | 1.36±0.06 |
| +Antimycin A | 0.84±0.1 | — |
| +Cyanide | 0.1±0.0 | — |
| Ascorbate/TMPD | 13.81±0.84 | 1.35±0.08 |
| +Rotenone | 13.69±0.81 | 1.33±0.04 |
| +Antimycin A | 13.72±0.81 | 1.30±0.02 |
| +Cyanide | 0.15±0.0 | — |
Oxygen consumption and ADP/O ratios for mitochondrial isolates from indicated plants. Substrates used are succinate and ascorbate/TMPD. Inhibitors used are rotenone, antimycin A, and cyanide (Associated with Fig. 5).