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. Author manuscript; available in PMC: 2016 Mar 1.
Published in final edited form as: Eur J Immunol. 2014 Dec 16;45(3):728–737. doi: 10.1002/eji.201444787

Figure 2. pDC in the thymus present an activated “IFN-α signature” phenotype.

Figure 2

A) Multicolor flow cytometry was performed on total thymocytes, fetal splenocytes and PBMC. pDC were gated as CD123+CD45RA+CD3−CD1a−CD4+ cells, and assessed for the expression of: ILT7, CD62L, BST2, CD31, BDCA2 and BDCA4. B) Mean percentages of positive cells ± SD from independent donors analyzed as in A. Thymus, n = 11; spleen, n = 4; PBMC, n = 4. C) Mean fold difference in MFI ± SD that were calculated as: MFI of antigen/MFI of IgG control [35] from: 11 thymus, 4 fetal spleen and 4 PBMC from independent donors analyzed as in A. *p<0.05, **p<0.01 as calculated using the Mann Whitney test.