Skip to main content
. Author manuscript; available in PMC: 2015 Jun 6.
Published in final edited form as: Neuroscience. 2014 Mar 26;269:67–78. doi: 10.1016/j.neuroscience.2014.03.014

Figure 2.

Figure 2

Dose-dependent effects of episodic intrathecal injections (3×5μl, 5 min intervals) of the nitric oxide (NO) donor sodium nitroprusside (SNP) on phrenic (A) and hypoglossal (XII) (B) nerve activity. Injections delivered to CSF via catheter placed near the phrenic motor nucleus (~C4–C5). Solid bars in A and B represent average values of integrated phrenic and XII nerve activity at 60 min post-SNP injections SNP-induced pMF occurred after 10μM injections, reaching a peak at 100μM. The highest SNP dose (1mM) failed to elicit pMF. *indicates significant difference from vehicle (i.e. time control, TC); #indicates significant difference from 1mM treated rats. Representative phrenic and XII neurograms from individual rats that received episodic intrathecal injections of (C) 100μM SNP or (D) vehicle (aCSF). In C and D, open arrows indicate timing of injections; solid arrows indicate blood sampling; horizontal dashed arrows at the end of each trace indicate baseline amplitude; grey region illustrates phrenic nerve activity exceeding baseline after SNP. (i.e. pMF). There was no SNP effect on XII nerve activity.