Table 3.
Strains and plasmids used in this study
| Strain/plasmid | Relevant phenotype/genotype | Reference |
|---|---|---|
| Strains | ||
| RP437 | Wild type for chemotaxis | 65 |
| EW388 | RP437ΔatpD | This study |
| EW405 | RP437ΔatpH | This study |
| EW389 | RP437ΔnuoCD | This study |
| DFB225 | RP437ΔfliG | 66 |
| BW25113 | Wild type | 67 |
| JW3805 | Wild type for chemotaxis; BW25113ΔmetE | 22 |
| JW3709 | BW25113ΔatpC (F1-ε) | 22 |
| JW3710 | BW25113ΔatpD (F1-β) | 22 |
| JW3711 | BW25113ΔatpG (F1-γ) | 22 |
| JW3712 | BW25113ΔatpA (F1-α) | 22 |
| JW3713 | BW25113ΔatpH (F1-δ) | 22 |
| JW3714 | BW25113ΔatpF (Fo-b) | 22 |
| JW3716 | BW25113ΔatpB (Fo-a) | 22 |
| EW390 | BW25113ΔatpE (Fo-c) | This study |
| EW379 | JW3710 + atpD | This study |
| EW380 | JW3710 + pCA24N | This study |
| RP1091 | RP437Δ(cheA cheW tar cheR cheB cheY cheZ) | 33 |
| EW267 | RP1091 + cheY | Fraiberg and Eisenbach, unpublished |
| EW385 | EW267ΔatpD | This study |
| EW386 | EW267ΔatpA | This study |
| EW387 | EW267ΔatpG | This study |
| MG1655 | Wild type for FRET measurements | 68 |
| VS116 | Δ flhC | 69 |
| Plasmids | ||
| pCA24N | Expression vector; CmR | 70 |
| atpD | β subunit expression plasmid; pCA24N derivative | 70 |
| cheY | CheY expression plasmid; pCA24N derivative | 70 |
| pBAD33 | Expression vector; pACYC ori, pBAD promoter, CmR | 71 |
| pDK2 | Expression vector for cloning of N-terminal CFPA206K fusions; pTrc99a derivative | 69 |
| pDK4 | Expression vector for cloning of N-terminal YFPA206K fusions; pTrc99a derivative | 69 |
| pDK66 | Expression vector for cloning of C-terminal YFPA206K fusions; pTrc99a derivative | 69 |
| pDK85 | Expression vector for cloning of C-terminal CFPA206K fusions; pTrc99a derivative | 69 |
| pDK113 | Expression vector for cloning of C-terminal CFPA206K fusions; pTrc99a derivative; start code of CFPA206K was changed from atg to gtg | 11 |
| pDK79 | Expression vector; pACYC ori, pBAD promoter, KanR | 69 |
| pEWBI | YFP-β expression plasmid; pDK4 derivative | This study |
| pEWB2 | β-YFP expression plasmid; pDK66 derivative | This study |
| pEWN1 | YFP-NuoCD expression plasmid; pDK4 derivative | This study |
| pEWN2 | NuoCD-YFP expression plasmid; pDK66 derivative | This study |
| pHL13 | FliN-CFP expression plasmid; pDK79 derivative | 11 |
| pHL15 | FliG-CFP expression plasmid; pDK79 derivative | 11 |
| pHL16 | CFP-FliN expression plasmid; pDK79 derivative | 11 |
| pHL50 | CFP-FliG expression plasmid; pDK79 derivative | 11 |
| pVS31 | CFP-FliM expression plasmid; pBAD33 derivative | 11 |
| pVS42 | FliM-CFP expression plasmid; pBAD33 derivative | 11 |