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. Author manuscript; available in PMC: 2016 Mar 2.
Published in final edited form as: Curr Protoc Pharmacol. 2015 Mar 2;68:2.12.1–2.12.26. doi: 10.1002/0471141755.ph0212s68

Fig. 4. Arrestin-3 promotes JNK1/2 activation induced by the expression of ASK1 in intact cells.

Fig. 4

A. Representative Western blot showing phosphorylation of endogenous JNK1/2 isoforms with or without arrestin-3 in COS-7 cells expressing varying amounts of ASK1. Upper p-JNK blot is the same as lower blot exposed for longer time to visualize p54 isoforms. B. Quantification of phosphorylation of JNK p46H and p54 isoforms with or without arrestin-3. ANCOVA with arrestin-3 as factor and ASK1 concentration as co-variate showed significant effect of ASK1 concentration on the level of p46H and p54 phosphorylation (p<0.0001). * - p<0.05, ** - p<0.01 to - Arr3, Student’s t-test for individual points. Data from (Kook et al., 2014)