Table 1. Preparing the kinase mix.

	Volume (each)	Volume (20 ×)
10× Kinase assay buffer	2 μl	40 μl
100 mM DTT	0.4 μl	8 μl
JNK3α2 *	0.8 μl	16 μl
p-MKK4 or p-MKK7 #	0.1 μl	2 μl
Total	3.3 μl	66 μl

^*JNK3α2 stock concentration: 12 μM;

^#p-MKK4 and p-MKK7 stock concentrations: 10 μM.