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. Author manuscript; available in PMC: 2016 Mar 2.
Published in final edited form as: Curr Protoc Pharmacol. 2015 Mar 2;68:2.12.1–2.12.26. doi: 10.1002/0471141755.ph0212s68

Table 10. Transfection to detect biphasic effect of arrestin-3 on MKK7-stimulated JNK phosphorylation (Fig. 6C,D).

pcDNA3-
Arrestin-3		 0 μg 0 μg 0.1 μg 0.25 μg 0.5 μg 1.0 μg 2.0 μg 4.0 μg 5.5 μg
pcDNA3-Flag-
MKK7		 0 μg 0.08 μg 0.08 μg 0.08 μg 0.08 μg 0.08 μg 0.08 μg 0.08 μg 0.08 μg
pcDNA3 5.58 μg 5.5 μg 5.4 μg 5.25 μg 5.0 μg 4.5 μg 3.5 μg 1.5 μg 0 μg
FuGENE HD 18μl 18μl 18μl 18μl 18μl 18μl 18μl 18μl 18μl
Opti-MEM 125μl 125μl 125μl 125μl 125μl 125μl 125μl 125μl 125μl
*

Note: See note to Table 9 above..