Table 3. Preparing the kinase mix (JNK1 or JNK2).

	Volume (each)	Volume (10 ×)
10× Kinase assay buffer	2 μl	20 μl
100 mM DTT	0.4 μl	4 μl
JNK1α1 or JNK2α2 *	0.8 μl	8 μl
p-MKK4 or p-MKK7 #	0.1 μl	1 μl
Total	3.3 μl	33 μl

^*Stock concentration: 12 μM