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. Author manuscript; available in PMC: 2016 Mar 2.
Published in final edited form as: Curr Protoc Pharmacol. 2015 Mar 2;68:2.12.1–2.12.26. doi: 10.1002/0471141755.ph0212s68

Table 5. Transfection to determine ASKl-dependent JNK activation.

pcDNA3-HA-ASK1 0 μg 0.05 μg 0.1 μg 0.25 μg 0.5 μg 1 μg 1.5 μg
pcDNA3 2.0 μg 1.95 μg 1.9 μg 1.75 μg 1.5 μg 1 μg 0.5 μg
FuGENE HD 6μl 6μl 6μl 6μl 6μl 6μl 6μl
Opti-MEM 125μl 125μl 125μl 125μl 125μl 125μl 125μl
*

Note: For MKK7-induced JNK activation, add pcDNA3-Flag-MKK7β1 instead of pcDNA3-HA-ASK1.