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| pcDNA3-HA-MKK4 | 0 μg | 0.2 μg | 0.5 μg | 1.0 μg | 2.0 μg | 4.0 μg |
| pcDNA3 | 4.5 μg | 4.3 μg | 4.0 μg | 3.5 μg | 2.5 μg | 0.5 μg |
| FuGENE HD | 13.5μl | 13.5μl | 13.5μl | 13.5μl | 13.5μl | 13.5μl |
| Opti-MEM | 125μl | 125μl | 125μl | 125μl | 125μl | 125μl |
