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. Author manuscript; available in PMC: 2015 Mar 16.
Published in final edited form as: Life Sci. 2012 Mar 3;91(0):613–617. doi: 10.1016/j.lfs.2012.02.016

Fig. 1. rEDNRB-KO mouse ovary is deficient of ENDRB gene and mRNA expression.

Fig. 1

A, Schematic diagram of EDNRB genomic structure of the WT and rEDNRB-KO mice. In the rEDNRB-KO mouse, exon 3 is replaced with Neo cassette and the genome has a transgenic insertion of DbH-promoter-EDNRB cDNA expression construct. B, Confirmation of absence of exon 3 in the rEDNRB-KO ovarian genomic DNA. Genomic DNA isolated from rEDNRB-KO and WT mice were used for genotyping by PCR. PCR with P3+P4 primer combination yields 298bp product from rEDNRB-KO transgenic construct, whereas 410bp product from WT genome. P1+P2 primer combination would give rise to 1,200 bp PCR product from rEDNRB-KO genome, but no product from WT genome. C, Detection of EDNRB mRNA. Total RNA was extracted and used for the mRNA detection by RT-PCR. EDNRB bands are shown in WT but not rEDNRB-KO ovaries. Two mice for each genotype were used. L19, and ribosomal RNA was used as internal control.