Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Mar 13;15:64. doi: 10.1186/s12866-015-0396-6

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© Garza-Ramos et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

PMC Copyright notice

Multiplex-PCR amplification of oligonucleotides combinations used for identification of K. variicola . A) Lane 1, molecular weight 1 kb; Lane 2, oligonucleotide combination named M-PCR-1 (KV770-F/KV770-R, KP888-F/KP888-R and KmtnC-F/KmtnC-R); Lane 3, oligonucleotide combination named M-PCR-2 (KV1615-F/KV1615-R, KP878-F/KP878-R and KmtnC-F/KmtnC-R); Lane 4, oligonucleotide combination named M-PCR-3 (KV1000-F/KV1000-F, KP888-F/KP888-R and KmtnC-F/KmtnC-R) (Table 1). B) M-PCR-1 assayed on environmental endophytic K. variicola isolates. Lane 1, molecular weight ΦX174 DNA-Hae III; Lane 2, K. pneumoniae ATCC 13883 and K. variicola 801, DNA’s combination; Lane 3, K. variicola T29A; Lane 4, K. variicola 6A2; Lane 5, K. variicola CFNE 2006; Lane 6, K. variicola 3; Lane 7, K. variicola F2R9; Lane 8, K. variicola VI; Lane 9, negative control (without DNA).