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. Author manuscript; available in PMC: 2015 Mar 16.
Published in final edited form as: Acc Chem Res. 2011 Jun 29;44(12):1312–1319. doi: 10.1021/ar2000474

Figure 3. X-ray footprinting of 30S ribosome assembly.

Figure 3

The evolution of RNA and RNA-protein interactions was probed from 20 ms to 120 s, using a 10 ms X-ray pulse.55 Native E. coli 16S rRNA was pre-folded in reconstitution buffer before addition of native E. coli 30S proteins (TP30). Quantitation of primer extension products revealed multi-stage folding of individual residues throughout the 16S rRNA.