Representative immunoblots and densitometric analysis of cystathionine beta synthase (CBS), cystathionine gamma lyase (CSE), and 3-mercaptopyruvate sulfutransferase (3-MST) (A-B). Circulating (C-D) and cardiac (E-F) levels of free hydrogen sulfide (H2S) and sulfane sulfure. All samples (blood and hearts) were collected from control, high-fat diet (HFD), and HFD-fed mice supplemented with SG-1002 (20 mg/kg/day). One group of mice received the SG-1002 supplemented HFD chow for the entire 24 weeks (HFD-S). We delayed the treatment in another group mice (HFD-D). These mice received the HFD chow for 12 weeks before being switched to the SG-1002 supplemented HFD chow for the final 12 weeks of the study. Results are expressed as mean ± SEM. Numbers in bars represent the sample size. *p<0.05, **p<0.01 and ***p<0.001 vs. Control.